doi: 10.1111/j.Ĭarrillo-Vico A., Calvo J.R., Abreu P., Lardone P.J., García-Mauriño S., Reiter R.J., Guerrero J.M. Melatonin: Nature’s most versatile biological signal? FEBS J. Pandi-Perumal S.R., Srinivasan V., Maestroni G.J.M., Cardinali D.P., Poeggeler B., Hardeland R. Distinct types of diffuse large B-cell lymphoma identified by gene expression profiling. doi: 10.1200/JCO.20.Īlizadeh A.A., Eisen M.B., Davis R.E., Ma C., Lossos I.S., Rosenwald A., Boldrick J.C., Sabet H., Tran T., Yu X., et al. Diffuse large B-cell lymphoma: Optimizing outcome in the context of clinical and biologic heterogeneity. Our findings confirm that MLT is a promising in vitro anti-tumour agent that requires further evaluation when used with other drugs active against DLBCL.Īpoptosis cell cycle dexamethasone diffuse large B-cell lymphoma doxorubicin melatonin. Furthermore, we observed increased cytotoxic and pro-apoptotic activity as well as the modulation of the cell cycle after the combination of MLT with DOX, DEX, or a combination of DOX + DEX, compared with both drugs or MLT used alone. The pro-apoptotic effect was exerted through both the mitochondrial and caspase-dependent pathways. MLT inhibited cell viability as well as induced apoptosis and cell cycle arrest at the G0/G1 phase. Additionally, to elucidate the mechanisms of action, caspase-3, -8, and -9 and a decline in the mitochondrial potential were determined using flow cytometry. The cytotoxicity and cell cycle distribution were measured using propidium iodide staining, while apoptosis was assessed using the annexin-V binding method. The aim of this study was to assess the cytotoxicity and apoptosis of MLT, used alone or in combination with one of the most active anti-cancer drugs, doxorubicin (DOX), and a well-known anti-inflammatory drug, dexamethasone (DEX), on a diffuse large B-cell lymphoma (DLBCL)-derived cell line. MLT is of great interest as a natural substance with anti-cancer activities. Melatonin (MLT), a pineal gland hormone, not only regulates circadian and seasonal rhythms, but also plays an important role in many aspects of human physiology and pathophysiology.
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